|In charge||Biophotonic||Flow adhesion||Flow cytometry|
The adhesion platform in dynamic and microfluidic flow described below is under the responsibility of the INTS research engineer – activity manager whose missions are the provision of analysis, training, advice and assistance the development of specific applications in microscopy, microfluidics and image analysis.
Plateform of flow adhesion – microfluidic (Zeiss)
Fully motorized and controlled by AxioVision software (Zeiss), the Axio-Observer Z1 videomicroscope is equipped with a piezo motorized heated stage as well as a thermostatically controlled enclosure regulated in humidity and gas (CO2 and N) making it possible to carry out long term time lapses under normoxic or hypoxic conditions.
The Definite focus module allows the focus to be maintained whatever the experimental and environmental conditions.
The illumination sources consist of an HXP120 box and the Colibri system (365, 470, 555, 590 and 625nm including 4 diodes simultaneously).
The compatible objectives for fluorescence, transmission and differential interference contrast (DIC) are all APO Plan and of high numerical aperture: 10x / 0.45, 20x / 0.8, 40x / 0.95, 40x / 1.3 oil, 63x / 1.4 oil and 100x /1.4 oil.
Equipped with two AxioCam HSm cameras and a Dual cam module, it is possible to image 60 images / s per color without time distortion between the two fluorescences. A third, more sensitive AxioCam MRm camera equips the microscope entirely controlled by a TFT touch screen.
This flow adhesion platform mimics physiological blood flow (shear force) inside the microcapillary vessels, in normoxic or hypoxic conditions.
This instrument was funded by the Ile-de-France Region (SESAME), INTS, Inserm and Paris Diderot University.