Biophotonic Imaging

 

All the instruments listed below are under the responsibility of an INTS engineer – activity manager and an INTS technician whose missions are the provision of analysis, training, advice and help in the development of specific applications in microscopy and image analysis.

Confocal microscope C1 (Nikon)

Crédit : J.PICOT

Equipped with a heating plate as well as a thermostatically controlled enclosure regulated in humidity and gas, the confocal microscope C1 (Eclipse TE300 stand) allows imaging of subcellular elements or the migration, division and differentiation of cells in culture on the long term. It is equipped with filter blocks allowing to observe fluorochromes such as DAPI, GFP and TRITC at various magnifications (2x / 0.06 Plan; 4x / 0.13 Plan; 10x / 0.45 DIC Plan; 20x / 0.75 DIC Plan; 40x / 0.75 DIC Plan ; 60x / 1.4 DIC Plan APO oil; 100x / 1.3 DIC Plan APO oil). The microscope is equipped for differential interference contrast (DIC) imaging. The laser bench consists of a 100mW Argon 488nm laser and a 4mW HeNe 543nm laser.

This instrument was funded by INTS, Inserm and Paris Diderot University.

 

Axio-Observer Z1 videomicroscope (Zeiss)

Crédit : J.PICOT

Fully motorized and controlled by AxioVision software (Zeiss), the Axio-Observer Z1 videomicroscope is equipped with a piezo motorized heated stage as well as a thermostatically controlled enclosure regulated in humidity and gas (CO2 and N) making it possible to carry out long term time lapses under normoxic or hypoxic conditions.

The Definite focus module allows the focus to be maintained whatever the experimental and environmental conditions. The illumination sources are composed of an HXP120 box and the Colibri system (365, 470, 555, 590 and 625nm including 4 diodes simultaneously).

The compatible objectives for fluorescence, transmission and differential interference contrast (DIC) are all APO Plan and of high numerical aperture: 10x / 0.45, 20x / 0.8, 40x / 0.95, 40x / 1.3 oil, 63x / 1.4 oil and 100x /1.4 oil.

Equipped with two AxioCam HSm cameras and a Dual cam module, it is possible to image 60 images / s per color without time distortion between the two fluorescences. A third, more sensitive AxioCam MRm camera equips the microscope entirely controlled by a TFT touch screen.

This instrument was funded by the Ile-de-France Region (SESAME), INTS, Inserm and Paris Diderot University.

 

Eclipse Ti-S videomicroscope (Nikon)

Crédit : J.PICOT

The Eclispe Ti-S inverted microscope is equipped with a monochrome VF MediaCybernetics camera for imaging in transmitted light cells in culture (heating platinum) and in fluorescence (DAPI, GFP, Rhodamine) at various magnifications (2x / 0.06 Plan ; 4x / 0.13 Plan; 10x / 0.45 DIC Plan; 20x / 0.75 DIC Plan; 40x / 0.75 DIC Plan; 60x / 1.4 DIC Plan APO oil; 100x / 1.3 DIC Plan APO oil).

Tissue imaging after histological staining is also possible thanks to a liquid crystal filter positioned in front of the camera.

This instrument was funded by INTS and Inserm.

 

BioStation IM (Nikon)

Crédit : J.PICOT

The BioStation IM is a compact cell surveillance system with incubation which allows to carry out a wide range of short-term or long-term multi-positional time-lapse experiments, in particular for growth, differentiation, morphology and d protein expression, and this at various magnifications (20x, 40x and 80x). Consistent environmental control of temperature, humidity and gas concentrations is ensured. Phase contrast imaging can be combined with two fluorescences (GFP / Rhodamine) simultaneously.

This instrument was funded by INTS and Inserm.

 

Data analysis and reprocessing station

This analysis and reprocessing station contains AxioVision (Zeiss), ImageJ and Adobe Photoshop CS3 software allowing 2D and 3D deconvolution; segmentation; automatic counting based on size, shape and light intensity criteria; object tracking …